✎ publications [Molecular Microbiology and Infection Unit]

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====== Publications ======

===== 2019  =====
Pinho MD, Foster G, Pomba C, Machado MP, Baily JL, Kuiken T, [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]], Portuguese Group for the Study of Streptococcal Infections. 2019. <fc #0000FF>//Streptococcus canis// are a single population infecting multiple animal hosts despite the diversity of the universally present M-like protein SCM</fc>. Front Microbiol 10:631.[[https://doi.org/10.3389/fmicb.2019.00631|doi:10.3389/fmicb.2019.00631]] [[http://www.ncbi.nlm.nih.gov/pubmed/30984150|(PMID: 30984150)]]

[[people:anacosta|Silva-Costa C]], Brito MJ, Aguiar SI, Lopes JP, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections, Portuguese Study Group of Invasive Pneumococcal Disease of the Pediatric Infectious Disease Society. 2019. <fc #0000FF>Dominance of vaccine serotypes in pediatric invasive pneumococcal infections in Portugal (2012-2015)</fc>. Sci Rep 9:6. [[https://doi.org/10.1038/s41598-018-36799-x|doi:10.1038/s41598-018-36799-x]] [[http://www.ncbi.nlm.nih.gov/pubmed/30626918|(PMID: 30626918)]]

Echániz-Aviles G, Guerreiro SI, [[people:anacosta|Silva-Costa C]], [[people:cmendes|Mendes CI]], [[people:jcarrico|Carriço JA]], Carnalla-Barajas MN, Soto-Noguerón A, Velazquez-Meza ME, [[people:jmelocristino|Melo-Cristino J]], Luévanos-Velazquez A, Martínez-Medina L, Vázquez-Larios MDR, [[people:ramirez|Ramirez M]]. 2019. <fc #0000FF>//Streptococcus pneumoniae// serotype 3 in Mexico (1994-2017): decrease of the unusual CC4909 lineage post-PCV13 introduction</fc>. J Clin Microbiol. 57 e01354-18 [[https://doi.org/10.1128/JCM.01354-18|doi:10.1128/JCM.01354-18]] [[http://www.ncbi.nlm.nih.gov/pubmed/30404945|(PMID: 30404945)]]

Pinto M, González-Díaz A, [[people:mpmachado|Machado MP]], Duarte S, Vieira L, [[people:jcarrico|Carriço JA]], Marti S, Bajanca-Lavado MP, Gomes JP. 2019. <fc #0000FF>Insights into the population structure and pan-genome of //Haemophilus influenzae//</fc>. Infect Genet Evol. 67:126–135 [[http://www.ncbi.nlm.nih.gov/pubmed/30391557|(PMID: 30391557)]]

Jesus TF, [[people:bgoncalves|Ribeiro-Gonçalves B]], Silva DN, Bortolaia V, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. 2019. <fc #0000FF>Plasmid ATLAS: plasmid visual analytics and identification in high-throughput sequencing data</fc>. Nucleic Acids Res 47:D188–D194 [[http://www.ncbi.nlm.nih.gov/pubmed/30395323|(PMID: 30395323)]]

===== 2018  =====
Caneiras C, Lito L, Mayoralas-Alises S, Díaz-Lobato S, [[people:jmelocristino|Melo-Cristino J]], Duarte A. 2018. <fc #0000FF>Virulence and resistance determinants of //Klebsiella pneumoniae// isolated from a Portuguese tertiary university hospital centre over a 31-year period</fc>. Enferm Infecc Microbiol Clin. //In press// [[http://www.ncbi.nlm.nih.gov/pubmed/30553621|(PMID: 30553621)]]

Caneiras C, Calisto F, Jorge da Silva G, Lito L, [[people:jmelocristino|Melo-Cristino J]], Duarte A. 2018. <fc #0000FF>First description of colistin and tigecycline-resistant //Acinetobacter baumannii// producing KPC-3 carbapenemase in Portugal</fc>. Antibiotics 7. [[https://doi.org/10.3390/antibiotics7040096|doi:10.3390/antibiotics7040096]] [[http://www.ncbi.nlm.nih.gov/pubmed/30404152|(PMID: 30404152)]]

[[people:ahoracio|Horácio AN]], [[people:anacosta|Silva-Costa C]], Lopes E, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections. 2018. <fc #0000FF>Conjugate vaccine serotypes persist as major causes of non-invasive pneumococcal pneumonia in Portugal despite declines in serotypes 3 and 19A (2012-2015)</fc>. PLoS ONE 13:e0206912.[[https://doi.org/10.1371/journal.pone.0206912|doi:10.1371/journal.pone.0206912]] [[http://www.ncbi.nlm.nih.gov/pubmed/30388168|(PMID: 30388168)]]

Hänscheid T, Hardisty DW, Henriques SO. 2018. <fc #0000FF>The crisis in scientific publishing: a holistic perspective about background issues associated with predatory publishing</fc>. Acta Med Port 31:524–526. [[http://www.ncbi.nlm.nih.gov/pubmed/30387419|(PMID: 30387419)]]

[[people:cpato|Pato C]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]], [[people:afriaes|Friães A]], Portuguese Group for the Study of Streptococcal Infections. 2018. <fc #0000FF>//Streptococcus pyogenes// causing skin and soft tissue infections are enriched in the recently emerged //emm//89 clade 3 and are not associated with abrogation of CovRS</fc>. Front Microbiol 9:2372.[[http://www.ncbi.nlm.nih.gov/pubmed/30356787|(PMID: 30356787)]]

Björnsdóttir ES, [[people:emartins|Martins ER]], Erlendsdóttir H, Haraldsson G, [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]], Kristinsson KG. 2018. <fc #0000FF>Group B streptococcal neonatal and early infancy infections in Iceland 1976 - 2015</fc>. Pediatr Infect Dis J. //In press// [[http://www.ncbi.nlm.nih.gov/pubmed/30299423|(PMID: 30299423)]] [[https://doi.org/10.1097/INF.0000000000002214|doi:10.1097/INF.0000000000002214]]

Huizenga E, van der Ende J, Zwinkels N, Jimissa A, van der Ende-Bouwman C, van Rooijen R, Kargbo B, Agnandji ST, Hänscheid T, Goorhuis A, Grobusch MP. 2018. <fc #0000FF>A modified case definition to facilitate essential hospital care during Ebola outbreaks</fc>. Clin Infect Dis. //In press// [[http://www.ncbi.nlm.nih.gov/pubmed/30239602|(PMID: 30239602)]] [[https://doi.org/10.1093/cid/ciy798|doi:10.1093/cid/ciy798]]

Branco A, [[people:jmelocristino|Melo-Cristino J]]. 2018. <fc #0000FF>Extreme parasitemia in //P. falciparum// malaria</fc>. Blood 132:868. [[http://www.ncbi.nlm.nih.gov/pubmed/30139832|(PMID: 30139832)]]

Barreto JN, de Lourdes Monteiro M, da Luz Lima M, Valadas E, Hänscheid T. 2018. <fc #0000FF>Under-reporting of tuberculosis in Praia, Cape Verde: study reports doubtful results</fc>. Int J Tuberc Lung Dis 22:1106. [[http://www.ncbi.nlm.nih.gov/pubmed/30092879|(PMID: 30092879)]]

Branco A, Francisco D, Hänscheid T. 2018. <fc #0000FF>Is there a “normal” oxygen concentration for in vitro //Plasmodium// cultures?</fc> Trends Parasitol 34:811–812. [[http://www.ncbi.nlm.nih.gov/pubmed/30049603|(PMID: 30049603)]]


Hänscheid T, Grobusch MP. 2018. <fc #0000FF>Could antibiotic treatment of traveller’s diarrhoea even prolong elimination of pathogens?</fc> Travel Med Infect Dis. //In press// [[http://www.ncbi.nlm.nih.gov/pubmed/30030070|(PMID: 30030070)]]

Couto N, Schuele L, Raangs EC, [[people:mpmachado|Machado MP]], [[people:cmendes|Mendes CI]], Jesus TF, Chlebowicz M, Rosema S, [[people:ramirez|Ramirez M]],[[people:jcarrico|Carriço JA]], Autenrieth IB, Friedrich AW, Peter S, Rossen JW.<fc #0000FF> Critical steps in clinical shotgun metagenomics for the concomitant detection and typing of microbial pathogens</fc>. Sci Rep. 2018 Sep 13;8(1):13767. doi: 10.1038/s41598-018-31873-w.[[http://www.ncbi.nlm.nih.gov/pubmed/30213965 |(PMID: 30213965)]]

Zhou Z, Alikhan NF, Sergeant MJ, Luhmann N, Vaz C, Francisco AP, [[people:jcarrico|Carriço JA]], Achtman M.<fc #0000FF> GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. </fc> Genome Res. 2018 Sep;28(9):1395-1404. doi: 10.1101/gr.232397.117. Epub 2018 Jul 26. [[http://www.ncbi.nlm.nih.gov/pubmed/30049790|(PMID: 30049790)]]

Teixeira AS, Monteiro PT, [[people:jcarrico|Carriço JA]], Santos FC, Francisco AP. 2018. <fc #0000FF>Large-scale simulations of bacterial populations over complex networks</fc>. J Comput Biol 25:850–861. [[http://www.ncbi.nlm.nih.gov/pubmed/29985650|(PMID: 29985650)]]

[[people:anacosta|Silva-Costa C]], Brito MJ, [[people:mdpinho|Pinho MD]], [[people:afriaes|Friães A]], Aguiar SI, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections, Portuguese Study Group of Invasive Pneumococcal Disease of the Pediatric Infectious Disease Society. 2018. <fc #0000FF>Pediatric complicated pneumonia caused by //Streptococcus pneumoniae// serotype 3 in 13-valent pneumococcal conjugate vaccinees, Portugal, 2010-2015</fc>. Emerging Infect Dis 24: 1307–1314. [[http://www.ncbi.nlm.nih.gov/pubmed/29912700|(PMID: 29912700)]]

Grobusch MP, Schaumburg F, Weitzel T, Rothe C, Hänscheid T, Goorhuis A. 2018. <fc #0000FF>Ebola 2018 - Implications for travel health advice and relevance for travel medicine</fc>. Travel Med Infect Dis 24:1–3. [[http://www.ncbi.nlm.nih.gov/pubmed/29906571|(PMID: 29906571)]]

Lopes E, Fernandes T, [[people:mpmachado|Machado MP]], [[people:jcarrico|Carriço JA]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]], [[people:emartins|Martins ER]], The Portuguese Group For The Study Of Streptococcal Infections. <fc #0000FF>Increasing macrolide resistance among //Streptococcus agalactiae// causing invasive disease in non-pregnant adults was driven by a single capsular-transformed lineage, Portugal, 2009 to 2015</fc>. Euro Surveill. 2018;23. doi:10.2807/1560-7917.ES.2018.23.21.1700473 [[http://www.ncbi.nlm.nih.gov/pubmed/29845930|(PMID: 29845930)]]

Hänscheid T, Hardisty DW. 2018. <fc #0000FF>How “resistant” is artemisinin resistant malaria? - The risks of ambiguity using the term “resistant” malaria</fc>. Travel Med Infect Dis 24:23–24. [[http://www.ncbi.nlm.nih.gov/pubmed/29715560|(PMID: 29715560)]]

Motro Y, [[people:jcarrico|Carriço JA]], Friedrich AW, Rossen JW, Moran-Gilad J. 2018.<fc #0000FF> ESCMID Postgraduate Education Course Regional Capacity Building for Integration of Next-Generation Sequencing in the Clinical Microlab.</fc> Microbes Infect 20:275–280. [[http://www.ncbi.nlm.nih.gov/pubmed/29555344 |(PMID: 29555344)]]

Silva M, [[people:mpmachado|Machado MP]], Silva DN, Rossi M, Moran-Gilad J, Santos S, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. <fc #0000FF> chewBBACA: A complete suite for gene-by-gene schema creation and strain identification.</fc> Microb Genom. 2018 Mar 15. doi: 10.1099/mgen.0.000166. [Epub ahead of print] [[http://www.ncbi.nlm.nih.gov/pubmed/29543149 |(PMID: 29543149)]]

[[people:jcarrico|Carriço JA]], Crochemore M, Francisco AP, Pissis SP, [[people:bgoncalves|Ribeiro-Gonçalves B]], Vaz C.<fc #0000FF> Fast phylogenetic inference from typing data.</fc> Algorithms Mol Biol. 2018 Feb 15;13:4. doi: 10.1186/s13015-017-0119-7. eCollection 2018. [[http://www.ncbi.nlm.nih.gov/pubmed/29467814 |(PMID: 29467814)]] 

Culebro A, [[people:mpmachado|Machado MP]], [[people:jcarrico|Carriço JA]], Rossi M. <fc #0000FF>Origin, evolution, and distribution of the molecular machinery for biosynthesis of sialylated lipooligosaccharide structures in //Campylobacter coli//.</fc> Sci Rep. 2018 Feb 14;8(1):3028. doi: 10.1038/s41598-018-21438-2.[[http://www.ncbi.nlm.nih.gov/pubmed/29445215 |(PMID: 29445215)]] 

[[people:jcarrico|Carriço JA]], Rossi M, Moran-Gilad J, Van Domselaar G, [[people:ramirez|Ramirez M]].<fc #0000FF> 2018. A Primer on Microbial Bioinformatics for non-bioinformaticians.</fc> Clin Microbiol Infect. 24:342–349.[[http://www.ncbi.nlm.nih.gov/pubmed/29309933 |(PMID: 29309933)]]

Savelkoel J, Binnendijk KH, Spijker R, van Vugt M, Tan K, Hänscheid T, Schlagenhauf P, Grobusch MP. 2018. <fc #0000FF>Abbreviated atovaquone-proguanil prophylaxis regimens in travelers after leaving malaria-endemic areas: A systematic review</fc>. Travel Med Infect Dis. 21:3–20. [[http://www.ncbi.nlm.nih.gov/pubmed/29242073|(PMID: 29242073)]]


===== 2017  =====
Hänscheid T, Grobusch MP. 2017. <fc #0000FF>Modern hematology analyzers are very useful for diagnosis of malaria and, crucially, may help avoid misdiagnosis</fc>. J Clin Microbiol 55:3303–3304. [[http://www.ncbi.nlm.nih.gov/pubmed/29066565|(PMID: 29066565)]]

Guedes LC, Chan RB, Gomes MA, Conceição VA, Machado RB, Soares T, Xu Y, Gaspar P, [[people:jcarrico|Carriço JA]], Alcalay RN, Ferreira JJ, Outeiro TF, Miltenberger-Miltenyi G. <fc #0000FF>Serum lipid alterations in GBA-associated Parkinson's disease </fc>. Parkinsonism Relat Disord. 2017 Sep 1. pii: S1353-8020(17)30322-X. [[http://www.ncbi.nlm.nih.gov/pubmed/28890071 |(PMID: 28890071)]]

Ribeiro TG, [[people:bgoncalves|Ribeiro-Gonçalves B]], da Silva MS, Novais A, Machado E, [[people:jcarrico|Carriço JA]], Peixe L. <fc #0000FF>//Citrobacter portucalensis// sp. nov., isolated from an aquatic sample </fc>. Int J Syst Evol Microbiol. 2017 Sep;67(9):3513-3517. [[http://www.ncbi.nlm.nih.gov/pubmed/28857032|(PMID: 28857032)]]

Jamet A, Touchon M, [[people:bgoncalves|Ribeiro-Gonçalves B]], [[people:jcarrico|Carriço JA]], Charbit A, Nassif X, [[people:ramirez|Ramirez M]], Rocha EPC. 2017. <fc #0000FF>A widespread family of polymorphic toxins encoded by temperate phages</fc>. BMC Biol 15:75. [[http://www.ncbi.nlm.nih.gov/pubmed/28851366|(PMID: 28851366)]]

Taboada EN, Graham MR, [[people:jcarrico|Carriço JA]], Van Domselaar G. 2017. <fc #0000FF>Food safety in the age of next generation sequencing, bioinformatics, and open data access</fc>. Front Microbiol 8:909. [[http://www.ncbi.nlm.nih.gov/pubmed/28588568|(PMID: 28588568)]]

Gil J, Almeida S, Constant C, Pinto S, Barreto R, [[people:jmelocristino|Melo-Cristino J]], Machado M do C, Bandeira T. 2017. <fc #0000FF>Short-term relevance of lower respiratory viral coinfection in inpatients under 2 years of age</fc>. An Pediatr //In press// [[http://www.ncbi.nlm.nih.gov/pubmed/28571896|(PMID: 28571896)]]

Visser BJ, de Vries SG, Vingerling R, Gritter M, Kroon D, Aguilar LC, Kraan RBJ, Wieten RW, Danion F, Sjouke B, Adegnika AA, Agnandji ST, Kremsner PG, Hänscheid T, Mens PF, van Vugt M, Grobusch MP. 2017. <fc #0000FF>Serum lipids and lipoproteins during uncomplicated malaria: A cohort study in Lambaréné, Gabon</fc>. Am J Trop Med Hyg 96:1205–1214. [[http://www.ncbi.nlm.nih.gov/pubmed/28500816|(PMID: 28500816)]]

[[people:emartins|Martins ER]], Pedroso-Roussado C, [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]], Portuguese Group for the Study of Streptococcal Infections. 2017. <fc #0000FF>//Streptococcus agalactiae// causing neonatal infections in Portugal (2005-2015): diversification and emergence of a CC17/PI-2b multidrug resistant sublineage</fc>. Front Microbiol 8:499. [[http://www.ncbi.nlm.nih.gov/pubmed/28400757|(PMID: 28400757)]]

Marchaim D, Levit D, Zigron R, Gordon M, Lazarovitch T, [[people:jcarrico|Carriço JA]], Chalifa-Caspi V, Moran-Gilad J. 2017. <fc #0000FF>Clinical and molecular epidemiology of //Acinetobacter baumannii// bloodstream infections in an endemic setting</fc>. Future Microbiol 12:271–283. [[http://www.ncbi.nlm.nih.gov/pubmed/28287300|(PMID: 28287300)]]

Henriques AR, [[people:jmelocristino|Melo-Cristino J]], Fraqueza MJ. 2017. <fc #0000FF>Genetic characterization of //Listeria monocytogenes// isolates from industrial and retail ready-to-eat meat-based foods and their relationship with clinical strains from human listeriosis in Portugal</fc>. J Food Prot 551–560. [[http://www.ncbi.nlm.nih.gov/pubmed/28272920|(PMID: 28272920)]]

[[people:jmiranda|Diamantino-Miranda J]], [[people:siaguiar|Aguiar, SI]], [[people:jcarrico|Carriço JA]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]]. 2017. <fc #0000FF>Clonal and serotype dynamics of serogroup 6 isolates causing invasive pneumococcal disease in Portugal: 1999-2012</fc>. PLoS ONE 12:e0170354. [[http://www.ncbi.nlm.nih.gov/pubmed/28152029|(PMID: 28152029)]]

[[people:mpmachado|Machado MP]], [[people:bgoncalves|Ribeiro-Gonçalves B]], Silva M, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. 2017. <fc #0000FF>Epidemiological surveillance and typing methods to track antibiotic resistant strains using high throughput sequencing</fc>. Methods Mol Biol 1520:331–355. [[http://www.ncbi.nlm.nih.gov/pubmed/27873262|(PMID: 27873262)]]

Nascimento M, Sousa A, [[people:ramirez|Ramirez M]], Francisco AP, [[people:jcarrico|Carriço JA]], Vaz C. 2017. <fc #0000FF>PHYLOViZ 2.0: Providing scalable data integration and visualization for multiple phylogenetic inference methods</fc>. Bioinformatics 33:128–129. [[http://www.ncbi.nlm.nih.gov/pubmed/27605102|(PMID: 27605102)]]



===== 2016  =====
[[people:ahoracio|Horácio AN]], [[people:anacosta|Silva-Costa C]], Lopes JP, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections. 2016. <fc #0000FF>Serotype 3 remains the leading cause of invasive pneumococcal disease in adults in Portugal (2012-2014) despite continued reductions in other 13-valent conjugate vaccine serotypes</fc>. Front Microbiol 7:1616. [[http://www.ncbi.nlm.nih.gov/pubmed/27790208|(PMID: 27790208)]]

Barato P, [[people:emartins|Martins ER]], Vasquez GM, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Martínez N, Iregui C. 2016. <fc #0000FF>Capsule impairs efficient adherence of //Streptococcus agalactiae// to intestinal epithelium in tilapias //Oreochromis// sp</fc>. Microb Pathog. 100:30–36. [[http://www.ncbi.nlm.nih.gov/pubmed/27594667|(PMID: 27594667)]]

[[people:mdpinho|Pinho MD]], Erol E, [[people:bgoncalves|Ribeiro-Gonçalves B]], [[people:cmendes|Mendes CI]], [[people:jcarrico|Carriço JA]], Matos SC, Preziuso S, Luebke-Becker A, Wieler LH, [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]]. 2016. <fc #0000FF>Beta-hemolytic //Streptococcus dysgalactiae// strains isolated from horses are a genetically distinct population within the //Streptococcus dysgalactiae// taxon</fc>. Sci Rep 6:31736. [[http://www.ncbi.nlm.nih.gov/pubmed/27530432|(PMID: 27530432)]]

Costa SS, Palma C, Kadlec K, Fessler AT, Viveiros M, [[people:jmelocristino|Melo-Cristino J]], Schwarz S, Couto I. 2016. <fc #0000FF>Plasmid-borne antimicrobial resistance of //Staphylococcus aureus// isolated in a hospital in Lisbon, Portugal</fc>. Microb Drug Resist. 22:617–626. [[http://www.ncbi.nlm.nih.gov/pubmed/27309487|(PMID: 27309487)]]

Mottola C, Matias CS, Mendes JJ, [[people:jmelocristino|Melo-Cristino J]], Tavares L, Cavaco-Silva P, Oliveira M. 2016. <fc #0000FF>Susceptibility patterns of //Staphylococcus aureus// biofilms in diabetic foot infections</fc>. BMC Microbiol 16:119.[[http://www.ncbi.nlm.nih.gov/pubmed/27339028|(PMID: 27339028)]]

[[people:ahoracio|Horácio AN]], [[people:anacosta|Silva-Costa C]], [[people:jmiranda|Diamantino-Miranda J]], Lopes JP, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections. 2016. <fc #0000FF>Population structure of //Streptococcus pneumoniae// causing invasive disease in adults in Portugal before PCV13 availability for adults: 2008-2011</fc>. PLoS ONE 11:e0153602. [[http://www.ncbi.nlm.nih.gov/pubmed/27168156|(PMID: 27168156)]]

[[people:bgoncalves|Ribeiro-Gonçalves B]], Francisco AP, Vaz C, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. 2016. <fc #0000FF>PHYLOViZ Online: web-based tool for visualization, phylogenetic inference, analysis and sharing of minimum spanning trees</fc>. Nucleic Acids Res. 44:W246-251. [[http://www.ncbi.nlm.nih.gov/pubmed/27131357|(PMID: 27131357)]]

Vieira S, Belo S, Hänscheid T. 2016. <fc #0000FF>Ziehl-Neelsen in schistosomiasis:much more than staining the shell and species identification</fc>. Am J Trop Med Hyg 94:699–700. [[http://www.ncbi.nlm.nih.gov/pubmed/27053189|(PMID: 27053189)]]

Rebelo M, Hänscheid T. 2016. <fc #0000FF>Flow Cytometry for Antimalarial Drug Testing: More than Meets the Eye</fc>. J Clin Microbiol 54:817. [[http://www.ncbi.nlm.nih.gov/pubmed/26917459|(PMID: 26917459)]]

Mottola C, Semedo-Lemsaddek T, Mendes JJ, [[people:jmelocristino|Melo-Cristino J]], Tavares L, Cavaco-Silva P, Oliveira M. 2016. <fc #0000FF>Molecular typing, virulence traits and antimicrobial resistance of diabetic foot staphylococci</fc>. J Biomed Sci 23:33. [[http://www.ncbi.nlm.nih.gov/pubmed/26952716|(PMID: 26952716)]]

Rebelo M, Grenho R, Orban A, Hänscheid T. 2016. <fc #0000FF>Transdermal diagnosis of malaria using vapor nanobubbles</fc>. Emerging Infect Dis 22:343–344. [[http://www.ncbi.nlm.nih.gov/pubmed/26811926|(PMID: 26811926)]]

Hänscheid T, Carvalho T, Grobusch MP. 2016. <fc #0000FF>Hemozoin detection for human malaria diagnosis investigated in rodent models: how similar is similar?</fc> Trends Parasitol. 32: 94–96 [[http://www.ncbi.nlm.nih.gov/pubmed/26704075|(PMID: 26704075)]]

Pires D, Zagalo A, Santos C, Cota de Medeiros F, Duarte A, Lito L, [[people:jmelocristino|Melo-Cristino J]], Caldeira L. 2016. <fc #0000FF>Evolving epidemiology of carbapenemase-producing Enterobacteriaceae in Portugal: 2012 retrospective cohort at a tertiary hospital in Lisbon</fc>. J Hosp Infect 92:82–85. [[http://www.ncbi.nlm.nih.gov/pubmed/26698390|(PMID: 26698390)]]

Björnsdóttir ES, [[people:emartins|Martins ER]], Erlendsdóttir H, Haraldsson G, [[people:jmelocristino|Melo-Cristino J]], Kristinsson KG, [[people:ramirez|Ramirez M]]. 2016.  <fc #0000FF>Changing epidemiology of group B streptococcal infections among adults in Iceland: 1975-2014</fc>. Clin Microbiol Infect. 22:379.e9-379.e16. [[http://www.ncbi.nlm.nih.gov/pubmed/26691681|(PMID: 26691681)]]
===== 2015  =====
[[people:afriaes|Friães A]], [[people:mpmachado|Machado MP]], [[people:cpato|Pato C]], [[people:jcarrico|Carriço JA]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]]. 2015. <fc #0000FF>Emergence of the same successful clade among distinct populations of //emm//89 //Streptococcus pyogenes// in multiple geographic regions</fc>. MBio 6. pii: e01780-15. [[http://www.ncbi.nlm.nih.gov/pubmed/26628724|(PMID: 26628724)]]
(Highlighted in Holzman DC. 2016. To outcompete others, //S. pyogenes// clade undergoes transmissibility upgrade. [[http://www.asmscience.org/content/journal/microbe/10.1128/microbe.11.56.1|Microbe 11:56-57]])

Costa SS, Viveiros M, Rosato AE, [[people:jmelocristino|Melo-Cristino J]], Couto I. 2015. <fc #0000FF>Impact of efflux in the development of multidrug resistance phenotypes in //Staphylococcus aureus//</fc>. BMC Microbiol 15:232.[[http://www.ncbi.nlm.nih.gov/pubmed/26498754|(PMID: 26498754)]]

Tempera C, Franco R, Caro C, André V, Eaton P, Burke P, Hänscheid T. 2015. <fc #0000FF>Characterization and optimization of the haemozoin-like crystal (HLC) assay to determine Hz inhibiting effects of anti-malarial compounds</fc>. Malar J 14:403. [[http://www.ncbi.nlm.nih.gov/pubmed/26458401|(PMID: 26458401)]]

Barreira R, Graça AM, Gonçalves C, Abrantes M, Gouveia R, Carvalho D, Lito L, Moniz C, [[people:jmelocristino|Melo-Cristino J]], Machado MC. 2015. <fc #0000FF>Monitorização epidemiológica numa unidade de cuidados intensivos neonatais</fc>. Acta Pediátrica Portuguesa 46:190–7. [[http://actapediatrica.spp.pt/article/view/7128|Publisher]]

Arinto-Garcia R, [[people:mdpinho|Pinho MD]], [[people:jcarrico|Carriço JA]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]]. 2015. <fc #0000FF>Comparing MALDI-TOF MS, phenotypic and molecular methods for the identification of species within the Streptococcus anginosus group</fc>. J Clin Microbiol. 53:3580–3588. [[http://www.ncbi.nlm.nih.gov/pubmed/26354817|(PMID: 26354817)]]

[[people:afriaes|Friães A]], [[people:cpato|Pato C]], [[people:jmelocristino|Melo-Cristino J]], [[people:ramirez|Ramirez M]]. 2015. <fc #0000FF>Consequences of the variability of the CovRS and RopB regulators among //Streptococcus pyogenes// causing human infections</fc>. Sci Rep 5:12057. [[http://www.ncbi.nlm.nih.gov/pubmed/26174161|(PMID: 26174161)]]

[[people:anacosta|Silva-Costa C]], [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections. 2015. <fc #0000FF>Declining macrolide resistance in //Streptococcus pyogenes// in Portugal (2007-13) was accompanied by continuous clonal changes</fc>. J Antimicrob Chemother. 70:2729–2733. [[http://www.ncbi.nlm.nih.gov/pubmed/26142474|(PMID: 26142474)]]

Coutinho CP, Barreto C, Pereira L, Lito L, [[people:jmelocristino|Melo-Cristino J]], Sá-Correia I. 2015. <fc #0000FF>Incidence of Burkholderia contaminans at a cystic fibrosis centre with an unusually high representation of Burkholderia cepacia during 15 years of epidemiological surveillance</fc>. J Med Microbiol 64:927–935. [[http://www.ncbi.nlm.nih.gov/pubmed/26002942|(PMID: 26002942)]]

[[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]], van der Linden M, [[people:jmelocristino|Melo-Cristino J]]. 2015. <fc #0000FF>Molecular Epidemiology of //Streptococcus pneumoniae//</fc>, p. 3-19 . In Brown, JM, Hammerschmidt, S, Orihuela, C (eds.), //Streptococcus Pneumoniae// - Molecular Mechanisms of Host-Pathogen Interactions. Academic Press, San Diego. [[http://dx.doi.org/10.1016/B978-0-12-410530-0.00001-6|DOI: 10.1016/B978-0-12-410530-0.00001-6]]

Janssen S, Hermans S, Knap M, Moekotte A, Rossatanga EG, Adegnika AA, Bélard S, Hänscheid T, Grobusch MP. 2015. <fc #0000FF>Impact of Anti-Retroviral Treatment and Cotrimoxazole Prophylaxis on Helminth Infections in HIV-Infected Patients in Lambaréné, Gabon</fc>. PLoS Negl Trop Dis 9:e0003769. [[http://www.ncbi.nlm.nih.gov/pubmed/25993501|(PMID: 25993501)]]

Rosini R, Campisi E, De Chiara M, Tettelin H, Rinaudo D, Toniolo C, Metruccio M, Guidotti S, Sørensen UBS, Kilian M, DEVANI Consortium, [[people:ramirez|Ramirez M]], Janulczyk R, Donati C, Grandi G, Margarit I. 2015. <fc #0000FF>Genomic analysis reveals the molecular basis for capsule loss in the group B streptococcus population</fc>. PLoS ONE 10:e0125985. [[http://www.ncbi.nlm.nih.gov/pubmed/25946017|(PMID: 25946017)]]

Rebelo M, Tempera C, Fernandes JF, Grobusch MP, Hänscheid T. 2015. <fc #0000FF>Assessing anti-malarial drug effects ex vivo using the haemozoin detection assay</fc>. Malar J 14:140. [[http://www.ncbi.nlm.nih.gov/pubmed/25879757|(PMID: 25879757)]]

Rebelo M, Tempera C, Bispo C, Andrade C, Gardner R, Shapiro HM, Hänscheid T. 2015. <fc #0000FF>Light depolarization measurements in malaria: A new job for an old friend</fc>. Cytometry A 87:437–445. [[http://www.ncbi.nlm.nih.gov/pubmed/25808846|(PMID: 25808846)]]

Teixeira AS, Monteiro PT, [[people:jcarrico|Carriço JA]], [[people:ramirez|Ramirez M]], Francisco AP. 2015. <fc #0000FF>Not seeing the forest for the trees: Size of the minimum spanning trees (MSTs) forest and branch significance in MST-based phylogenetic analysis.</fc> PLoS ONE 10:e0119315. [[http://www.ncbi.nlm.nih.gov/pubmed/25799056|(PMID: 25799056)]]

Coelho LP, [[people:cpato|Pato C]], [[people:afriaes|Friães A.]], Neumann A, von Köckritz-Blickwede M, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. 2015. <fc #0000FF>Automatic Determination of NET (Neutrophil Extracellular Traps) Coverage in Fluorescent Microscopy Images.</fc>
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[[people:anacosta|Silva-Costa C]], [[people:afriaes|Friães A]], [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]] 2015. <fc #0000FF>Macrolide-resistant //Streptococcus pyogenes//: prevalence and treatment strategies.</fc> Expert Rev Anti Infect Ther 13:615–628. [[http://www.ncbi.nlm.nih.gov/pubmed/25746210|(PMID: 25746210)]]

Barato P, [[people:emartins|Martins ER]], [[people:jmelocristino|Melo-Cristino J]], Iregui CA, [[people:ramirez|Ramirez M]]. 2015. <fc #0000FF>Persistence of a single clone of //Streptococcus agalactiae// causing disease in tilapia (//Oreochromis// sp.) cultured in Colombia over 8 years</fc>. J Fish Dis 38:1083–1087 [[http://www.ncbi.nlm.nih.gov/pubmed/25643734|(PMID: 25643734)]]

[[people:afriaes|Friães A]], C. Resina, V. Manuel, L. Lito, [[people:ramirez|Ramirez M]], and [[people:jmelocristino|Melo-Cristino J]]. 2015. <fc #0000FF>Epidemiological survey of the first case of vancomycin-resistant //Staphylococcus aureus// infection in Europe</fc>. Epidemiol Infect 143:745–8. [[http://www.ncbi.nlm.nih.gov/pubmed/24901752|(PMID: 24901752)]]

===== 2014  =====
Vaz C, Francisco AP, Silva M, Jolley KA, Bray JE, Pouseele H, Rothganger J, [[people:ramirez|Ramirez M]], [[people:jcarrico|Carriço JA]]. 2014. <fc #0000FF>TypOn: the microbial typing ontology</fc>. J Biomed Semantics 5:43. [[http://www.ncbi.nlm.nih.gov/pubmed/25584183|(PMID: 25584183)]]

Magalhães R, Ferreira V, Santos I, Almeida G, Teixeira P, Research Team. 2014. <fc #0000FF>Genetic and phenotypic characterization of //Listeria monocytogenes// from human clinical cases that occurred in Portugal between 2008 and 2012</fc>. Foodborne Pathog. Dis. 11:907–916. [[http://www.ncbi.nlm.nih.gov/pubmed/25361468|(PMID: 25361468)]]

Earnshaw S, Mancarella G, Mendez A, Todorova B, Magiorakos AP, Possenti E, Stryk M, Gilbro S, Goossens H, Albiger B, Monnet DL, European Antibiotic Awareness Day Technical Advisory Committee, European Antibiotic Awareness Day Collaborative Group. 2014. <fc #0000FF>European Antibiotic Awareness Day: a five-year perspective of Europe-wide actions to promote prudent use of antibiotics</fc>. Euro Surveill. 19: pii: 20928. [[http://www.ncbi.nlm.nih.gov/pubmed/25345519|(PMID: 25345519)]]

[[people:ramirez|Ramirez M]]. 2014. <fc #0000FF>//Streptococcus pneumoniae//</fc>, p. 1529–1546. //In// Tang, Y-W, Sussman, M, Liu, D, Poxton, I, Schwartzman, J (eds.), Molecular Medical Microbiology, 2nd ed. Academic Press, Elsevier.
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Hänscheid T, Rebelo M, Grobusch MP. 2014. <fc #0000FF>Point-of-care tests: where is the point?</fc> Lancet Infect Dis 14:922. [[http://www.ncbi.nlm.nih.gov/pubmed/25253406|(PMID: 25253406)]]

[[people:jcarrico|Carriço JA]], [[people:ramirez|Ramirez M]]. 2014. <fc #0000FF>Analysis of typing results</fc>, p. 85–112. //In// Oyarzabal, OA, Kathariou, S (eds.), DNA Methods in Food Safety: Molecular Typing of Foodborne and Waterborne Bacterial Pathogens. John Wiley & Sons, Ltd. [[http://onlinelibrary.wiley.com/doi/10.1002/9781118278666.ch5/summary|(DOI: 10.1002/9781118278666.ch5)]]

Branquinho R, Meirinhos-Soares L, [[people:jcarrico|Carriço JA]], Pintado M, Peixe LV. 2014 <fc #0000FF>Phylogenetic and clonality analysis of //Bacillus pumilus// isolates uncovered a highly heterogeneous population of different closely related species and clones.</fc> FEMS Microbiol Ecol. 2014 90:689–698. [[http://www.ncbi.nlm.nih.gov/pubmed/25230950|(PMID: 25230950)]]

Branquinho R, Sousa C, Osório H, Meirinhos-Soares L, Lopes J, [[people:jcarrico|Carriço J]], Busse H-J, Abdulmawjood A, Klein G, Kämpfer P, Pintado ME, Peixe L. 2014. <fc #0000FF>//Bacillus invictae sp. nov.//, isolated from health products in Portugal</fc>. Int. J. Syst. Evol. Microbiol. 64:3867–3876. [[http://www.ncbi.nlm.nih.gov/pubmed/25171924|(PMID: 25171924)]]

Neumann A, Völlger L, Berends ETM, Molhoek EM, Stapels DAC, Midon M, [[people:afriaes|Friães A]], Pingoud A, Rooijakkers SHM, Gallo RL, Mörgelin M, Nizet V, Naim HY, von Köckritz-Blickwede M. 2014. <fc #0000FF>Novel role of the antimicrobial peptide LL-37 in the protection of neutrophil extracellular traps against degradation by bacterial nucleases</fc>. J Innate Immun 6:860–868. [[http://www.ncbi.nlm.nih.gov/pubmed/25012862|(PMID: 25012862)]]

[[people:ahoracio|Horácio, AN]], Lopes JP, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], Portuguese Group for the Study of Streptococcal Infections. 2014. <fc #0000FF>Non-invasive pneumococcal pneumonia in Portugal - serotype distribution and antimicrobial resistance</fc>. PLoS ONE 9:e103092. [[http://www.ncbi.nlm.nih.gov/pubmed/25075961|(PMID: 25075961)]]

Hänscheid T, Gresnigt T, Löhr S, Flamen A, Zoller T, [[people:jmelocristino|Melo-Cristino J]], Grobusch MP. 2014. <fc #0000FF>Methaemoglobin and COHb in patients with malaria</fc>. Malar. J. 13:285. [[http://www.ncbi.nlm.nih.gov/pubmed/25056571|(PMID: 25056571)]]

Mendes JJ, Leandro C, Mottola C, Barbosa R, Silva FA, Oliveira M, Vilela CL, [[people:jmelocristino|Melo-Cristino J]], Górski A, Pimentel M, São-José C, Cavaco-Silva P, Garcia M. 2014. <fc #0000FF>In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections</fc>. J. Med. Microbiol. 63:1055–1065. [[http://www.ncbi.nlm.nih.gov/pubmed/24869663|(PMID: 24869663)]]

Orbán, A., Á. Butykai, A. Molnár, Z. Pröhle, G. Fülöp, T. Zelles, W. Forsyth, D. Hill, I. Müller, L. Schofield, M. Rebelo, T. Hänscheid, S. Karl, and I. Kézsmárki. 2014. <fc #0000FF>Evaluation of a novel magneto-optical method for the detection of malaria parasites</fc>. PLoS ONE 9:e96981. [[http://www.ncbi.nlm.nih.gov/pubmed/24824542|(PMID: 24824542)]]

Olafsdottir, L., H. Erlendsdottir, [[people:jmelocristino|J. Melo-Cristino]], D. Weinberger, [[people:ramirez|M. Ramirez]], K. Kristinsson, and M. Gottfredsson. 2014. <fc #0000FF>Invasive infections due to //Streptococcus pyogenes//: seasonal variation of severity and clinical characteristics, Iceland, 1975 to 2012</fc>. Euro Surveill. 19:pii: 20784 [[http://www.ncbi.nlm.nih.gov/pubmed/24821122|(PMID: 24821122)]]

Carrasco, M. P., A. S. Newton, L. Gonçalves, A. Góis, M. Machado, J. Gut, F. Nogueira, T. Hänscheid, R. C. Guedes, D. J. V. A. Dos Santos, P. J. Rosenthal, and R. Moreira. 2014. <fc #0000FF>Probing the aurone scaffold against //Plasmodium falciparum//: Design, synthesis and antimalarial activity</fc>. Eur J Med Chem 80:523–534. [[http://www.ncbi.nlm.nih.gov/pubmed/24813880|(PMID: 24813880)]]

Preziuso, S., [[people:mdpinho|M. D. Pinho]], A. R. Attili, [[people:jmelocristino|J. Melo-Cristino]], E. Acke, A. C. Midwinter, V. Cuteri, and [[people:ramirez|M. Ramirez]]. 2014. <fc #0000FF>PCR based differentiation between //Streptococcus dysgalactiae// subsp. //equisimilis// strains isolated from humans and horses</fc>. Comp. Immunol. Microbiol. Infect. Dis. 37(3): 169-72. [[http://www.ncbi.nlm.nih.gov/pubmed/24813401|(PMID: 24813401)]]

Sanderson-Smith M, De Oliveira DMP, Guglielmini J, McMillan DJ, Vu T, Holien JK, Henningham A, Steer AC, Bessen DE, Dale JB, Curtis N, Beall BW, Walker MJ, Parker MW, Carapetis JR, Van Melderen L, Sriprakash KS, Smeesters PR, The M Protein Study Group. 2014. <fc #0000FF>A systematic and functional classification of //Streptococcus pyogenes// that serves as a new tool for molecular typing and vaccine development</fc>. J Infect Dis 210:1325–1338. [[http://www.ncbi.nlm.nih.gov/pubmed/24799598|(PMID: 24799598)]]

López, E., A. Domenech, M.-J. Ferrándiz, [[people:mfrias|M. J. Frias]], C. Ardanuy, [[people:ramirez|M. Ramirez]], E. García, J. Liñares, and A. G. de la Campa. 2014. <fc #0000FF>Induction of prophages by fluoroquinolones in //Streptococcus pneumoniae//: implications for emergence of resistance in genetically-related clones</fc>. PLoS ONE 9:e94358. [[http://www.ncbi.nlm.nih.gov/pubmed/24718595|(PMID: 24718595)]]

[[people:siaguiar|Aguiar, SI]], Brito M, [[people:ahoracio|Horácio, AN]], Lopes J, [[people:ramirez|Ramirez M]], [[people:jmelocristino|Melo-Cristino J]], and Portuguese Group for the Study of Streptococcal Infections and the Portuguese Study Group of Invasive Pneumococcal Disease of the Paediatric Infectious Disease Society. 2014. <fc #0000FF>Decreasing incidence and changes in serotype distribution of invasive pneumococcal disease in persons aged under 18 years since introduction of 10-valent and 13-valent conjugate vaccines in Portugal, July 2008 to June 2012</fc>. Euro Surveill. 19:pii: 20750. [[http://www.ncbi.nlm.nih.gov/pubmed/24698140|(PMID: 24698140)]]

Carrolo, M, Pinto FR, [[people:jmelocristino|Melo-Cristino J]], and [[people:ramirez|Ramirez M]]. 2014. <fc #0000FF>Pherotype influences biofilm growth and recombination in //Streptococcus pneumoniae//</fc>. PLoS ONE 9:e92138. [[http://www.ncbi.nlm.nih.gov/pubmed/24646937|(PMID: 24646937)]]

Cardoso, L. S., C. E. Suissas, [[people:ramirez|M. Ramirez]], M. Antunes, and F. R. Pinto. 2014. <fc #0000FF>Comparison of alternative mixture model methods to analyze bacterial CGH experiments with multi-genome arrays</fc>. BMC Res Notes 7:148.[[http://www.ncbi.nlm.nih.gov/pubmed/24629208|(PMID: 24629208)]]

Moreira, A. S., C. P. Coutinho, P. Avezedo, L. Lito, [[people:jmelocristino|J. Melo-Cristino]], and I. Sá-Correia. 2014. <fc #0000FF>//Burkholderia dolosa// phenotypic variation during the decline in lung function of a cystic fibrosis patient along 5.5 years of chronic colonization</fc>. J. Med. Microbiol. 63:594–601. [[http://www.ncbi.nlm.nih.gov/pubmed/24469681|(PMID: 24469681)]]

[[people:anacosta|Silva-Costa, C. ]], [[people:jcarrico|J. A. Carriço]], [[people:ramirez|M. Ramirez]], and [[people:jmelocristino|J. Melo-Cristino]]. 2014. <fc #0000FF>Scarlet fever is caused by a limited number of //Streptococcus pyogenes// lineages and is associated with the exotoxin genes //ssa//, //speA// and //speC//</fc>. Pediatr Infect Dis J. 33(3):306-10. [[http://www.ncbi.nlm.nih.gov/pubmed/24168973|(PMID: 24168973)]]

Bajanca-Lavado MP, Simões AS, Betencourt CR, Sá-Leão R, Portuguese Group for Study of //Haemophilus influenzae// invasive infection. 2014. <fc #0000FF>Characteristics of //Haemophilus influenzae// invasive isolates from Portugal following routine childhood vaccination against //H. influenzae// serotype b (2002-2010)</fc>. Eur J Clin Microbiol Infect Dis 33:603–610. [[http://www.ncbi.nlm.nih.gov/pubmed/24154654|(PMID: 24154654)]]


===== 2013  =====
Costa, A., J. Afonso, C. Osório, A. L. Gomes, F. Caiado, J. Valente, [[people:siaguiar|S. I. Aguiar]], F. Pinto, [[people:ramirez|M. Ramirez]], S. Dias. 2013. <fc #0000FF>miR-363-5p regulates endothelial cell properties and their communication with hematopoietic precursor cells</fc>. J Hematol Oncol 6:87. [[http://www.ncbi.nlm.nih.gov/pubmed/24257019|(PMID: 24257019)]]

Faria, N. A., M. Miragaia, and H. de Lencastre, and the Multi Laboratory Project Collaborators. 2013. <fc #0000FF>Massive dissemination of methicillin resistant //Staphylococcus aureus// in bloodstream infections in a high mrsa prevalence country: establishment and diversification of EMRSA-15.</fc> Microb. Drug Resist. 19:483–490.
[[http://www.ncbi.nlm.nih.gov/pubmed/24171450|(PMID: 24171450)]]

[[people:ahoracio|Horácio, A. N.]], [[people:jmiranda|J. Diamantino-Miranda]], [[people:siaguiar|S. I. Aguiar]], [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]], and the Portuguese Group for the Study of Streptococcal Infections. 2013. <fc #0000FF>The majority of adult pneumococcal invasive infections in Portugal are still potentially vaccine preventable in spite of significant declines of serotypes 1 and 5.</fc> PLoS ONE 8:e73704. [[http://www.ncbi.nlm.nih.gov/pubmed/24066064|(PMID: 24066064)]]

Ressurreição, A. S., D. Gonçalves, A. R. Sitoe, I. S. Albuquerque, J. Gut, A. Góis, L. M. Gonçalves, M. R. Bronze, T. Hänscheid, G. A. Biagini, P. J. Rosenthal, M. Prudêncio, P. O’Neill, M. M. Mota, F. Lopes, and R. Moreira. 2013. <fc #0000FF>Structural optimization of Quinolon-4(1H)-imines as dual-stage antimalarials: toward increased potency and metabolic stability.</fc> J Med Chem. 56:7679–7690. [[http://www.ncbi.nlm.nih.gov/pubmed/23499920|(PMID: 23499920)]]

Boura, M., R. Frita, A. Góis, T. Carvalho, and T. Hänscheid. 2013. <fc #0000FF>The hemozoin conundrum: is malaria pigment immune-activating, inhibiting, or simply a bystander?</fc> Trends Parasitol. 29:469–476.  [[http://www.ncbi.nlm.nih.gov/pubmed/23937890|(PMID: 23937890)]]

[[people:afriaes|Friães A.]], [[people:jplopes|Lopes J.P.]], [[people:jmelocristino|Melo-Cristino J.]], [[people:ramirez|M. Ramirez]], the Portuguese Group for the Study of Streptococcal Infections. 2013. <fc #0000FF>Changes in //Streptococcus pyogenes// causing invasive disease in Portugal: Evidence for superantigen gene loss and acquisition</fc>. Int J Med Microbiol. 303:505-513 [[http://www.ncbi.nlm.nih.gov/pubmed/23932912|(PMID: 23932912)]]

Costa, S. S., E. Junqueira, C. Palma, M. Viveiros, [[people:jmelocristino|Melo-Cristino J.]] , L. Amaral, I. Couto. 2013. <fc #0000FF>Resistance to antimicrobials mediated by efflux pumps in //Staphylococcus aureus//</fc>. Antibiotics 2: 83-99, 2013. [[http://dx.doi.org/10.3390/antibiotics2010083|(doi:10.3390/antibiotics2010083)]]

Hanson, K. K., A. S. Ressurreição, K. Buchholz, M. Prudêncio, J. D. Herman-Ornelas, M. Rebelo, W. L. Beatty, D. F. Wirth, T. Hänscheid, R. Moreira, M. Marti, and M. M. Mota. 2013. <fc #0000FF>Torins are potent antimalarials that block replenishment of //Plasmodium// liver stage parasitophorous vacuole membrane proteins.</fc> Proc Natl Acad Sci USA. 110:E2838–2847. [[http://www.ncbi.nlm.nih.gov/pubmed/23836641|(PMID: 23836641)]]

Shapiro, H. M., S. H. Apte, G. M. Chojnowski, T. Hänscheid, M. Rebelo, and B. T. Grimberg. 2013. <fc #0000FF>Cytometry in malaria--a practical replacement for microscopy?</fc> Curr Protoc Cytom Chapter 11:Unit 11.20. [[http://www.ncbi.nlm.nih.gov/pubmed/23835802|(PMID: 23835802)]]

[[people:jmelocristino|Melo-Cristino J.]], C. Resina, V. Manuel, L. Lito, [[people:ramirez|M. Ramirez]]. 2013. <fc #0000FF>First case of infection with vancomycin-resistant //Staphylococcus aureus// in Europe.</fc> The Lancet. 382:205 [[http://www.ncbi.nlm.nih.gov/pubmed/23791472|(PMID: 23791472)]]

Mendes J.J., C. Leandro, S. Corte-Real, R. Barbosa, P. Cavaco-Silva, [[people:jmelocristino|J. Melo-Cristino]], A. Górski, M. Garcia. 2013. <fc #0000FF>Wound healing potential of topical bacteriophage therapy on diabetic cutaneous wounds.</fc> Wound Repair Regen. 21:595-603. [[http://www.ncbi.nlm.nih.gov/pubmed/23755910|(PMID: 23755910)]]

Rebelo, M., C. Sousa, H. M. Shapiro, M. M. Mota, M. P. Grobusch, and T. Hänscheid. 2013. <fc #0000FF>A novel flow cytometric hemozoin detection assay for real-time sensitivity testing of //Plasmodium falciparum//.</fc> PLoS ONE 8:e61606. [[http://www.ncbi.nlm.nih.gov/pubmed/23637865|(PMID: 23637865)]]

Tavares, A., M. Miragaia, J. Rolo, C. Coelho, H. de Lencastre, and CA-MRSA/MSSA working group. 2013. <fc #0000FF>High prevalence of hospital-associated methicillin-resistant //Staphylococcus aureus// in the community in Portugal: evidence for the blurring of community-hospital boundaries.</fc> Eur. J. Clin. Microbiol. Infect. Dis. 32:1269–1283. [[http://www.ncbi.nlm.nih.gov/pubmed/23604782|(PMID: 23604782)]]

Espadinha, D., N. A. Faria, M. Miragaia, L. M. Lito, [[people:jmelocristino|J. Melo-Cristino]], and H. de Lencastre. 2013. <fc #0000FF>Extensive dissemination of methicillin-resistant //Staphylococcus aureus// (MRSA) between the hospital and the community in a country with a high prevalence of nosocomial MRSA.</fc> PLoS ONE 8:e59960. [[http://www.ncbi.nlm.nih.gov/pubmed/23593155|(PMID: 23593155)]]

Gouveia, C., [[people:afriaes|A. Friães]], M. Cassiano-Neves, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2013. <fc #0000FF>MRSA and PVL positive //Staphylococcus aureus// are rarely found in community-acquired osteoarticular infections in children in Portugal, a country with high MRSA prevalence.</fc> Online Int J of Micr Res 1:20–24. [[http://www.onlineresearchjournals.org/OIJMR/abstracts/2013/mar/Gouveia%20et%20al..htm|(Online)]]

Vaz, M., L. Meirinhos-Soares, C. C. S. Sousa, [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]], J. A. Lopes. 2013. <fc #0000FF>Serotype discrimination of encapsulated //Streptococcus pneumoniae// strains by Fourier-transform infrared spectroscopy and chemometrics.</fc>  J. Microbiol. Methods 93:102–107. [[http://www.ncbi.nlm.nih.gov/pubmed/23499920|(PMID: 23499920)]]

Valadas, E., A. Gomes, A. Sutre, S. Brilha, A. Wete, T. Hänscheid, and F. Antunes. 2013. <fc #0000FF>Tuberculosis with malaria or HIV co-infection in a large hospital in Luanda, Angola.</fc> J Infect Dev Ctries 7:269–272. [[http://www.ncbi.nlm.nih.gov/pubmed/23493006|(PMID: 23493006)]]

McMillan, D. J., P.-A. Drèze, T. Vu, D. E. Bessen, J. Guglielmini, A. C. Steer, J. R. Carapetis, L. Van Melderen, K. S. Sriprakash, P. R. Smeesters and the M protein study group. 2013. <fc #0000FF>Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study</fc>. Clin. Microbiol. Infect. 19:E222–229. [[http://www.ncbi.nlm.nih.gov/pubmed/23464795|(PMID: 23464795)]]

Costa, S. S., C. Mourato, M. Viveiros, [[people:jmelocristino|J. Melo-Cristino]], L. Amaral, and I. Couto. 2013. <fc #0000FF>Description of plasmid pSM52, harbouring the gene for the Smr efflux pump, and its involvement in resistance to biocides in a meticillin-resistant //Staphylococcus aureus// strain.</fc> Int. J. Antimicrob. Agents. 41:490–492. [[http://www.ncbi.nlm.nih.gov/pubmed/23434536|(PMID: 23434536)]]

Coelho JR, [[people:jcarrico|Carriço JA]], Knight D, Martínez JL, Morrissey I, Oggioni MR, Freitas AT. 2013 <fc #0000FF>The use of machine learning methodologies to analyse antibiotic and biocide susceptibility in //Staphylococcus aureus//.</fc> PLoS One. 2013;8(2):e55582. doi: 10.1371/journal.pone.0055582. Epub 2013 Feb 19. [[http://www.ncbi.nlm.nih.gov/pubmed/23431361|(PMID: 23431361)]]

[[people:jcarrico|J. A. Carriço]], A. Sabat , A. Friederich, [[people:ramirez|M. Ramirez]], on behalf of the ESCMID Study Group for Epidemiological Markers (ESGEM). 2013. <fc #0000FF>Bioinformatics in bacterial molecular epidemiology and public health: databases, tools and the next-generation sequencing revolution.</fc> Euro Surveill. 2013. 18:pii=20382. [[http://www.ncbi.nlm.nih.gov/pubmed/23369390|(PMID: 23369390)]]

[[people:mdpinho|Pinho M. D.]], [[people:scmatos|S. C. Matos]], C. Pomba, A. Lübke-Becker, L. H. Wieler, S. Preziuso, [[people:jmelocristino|J. Melo-Cristino]], [[people:ramirez|M. Ramirez]]. 2013. <fc #0000FF>Multilocus sequence analysis of //Streptococcus canis// confirms the zoonotic origin of human infections and reveals genetic exchange with //Streptococcus dysgalactiae// subsp. //equisimilis//.</fc> J. Clin. Microbiol. 51:1099-109. [[http://www.ncbi.nlm.nih.gov/pubmed/23345291|(PMID: 23345291)]]

[[people:emartins|Martins E. R.]], A. Andreu, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2013. <fc #0000FF>Distribution of pilus islands in //Streptococcus agalactiae// causing human infections: insights into evolution and implication for vaccine development.</fc> Clin. Vaccine Immunol. 20:313–316. [[http://www.ncbi.nlm.nih.gov/pubmed/23269415|(PMID: 23269415)]]

Janssen, S., X. Padanilam, R. Louw, R. Mahanyele, G. Coetzee, T. Hänscheid, T. Leenstra, and M. P. Grobusch. 2013. <fc #0000FF>Sputum culture results to monitor MDR-TB patients during treatment: How many do we need?</fc> J. Clin. Microbiol. 51:644-6. [[http://www.ncbi.nlm.nih.gov/pubmed/23224098|(PMID: 23224098)]]

[[people:mfrias|Frias, M. J.]], [[people:jmelocristino|J. Melo-Cristino]], [[people:ramirez|M. Ramirez]]. 2013. <fc #0000FF>Export of the pneumococcal phage SV1 lysin requires choline-containing teichoic acids and is holin-independent.</fc> Mol Microbiol. 87:430–445. [[http://www.ncbi.nlm.nih.gov/pubmed/23171061|(PMID: 23171061)]]

[[people:afriaes|A. Friães]], Pinto FR, [[people:anacosta|C. Silva-Costa]], [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]] 2013. <fc #0000FF>Superantigen gene complement of //Streptococcus pyogenes// - relationship with other typing methods and short-term stability.</fc> Eur. J. Clin. Microbiol. Infect. Dis. 32:115-125. [[http://www.ncbi.nlm.nih.gov/pubmed/22936424|(PMID: 22936424)]]
===== 2012  =====
[[people:afriaes|A. Friães]], Pinto FR, [[people:anacosta|C. Silva-Costa]], [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]], The Portuguese Group for the Study of Streptococcal Infections. 2012. <fc #0000FF>Group A streptococci clones associated with invasive infections and pharyngitis in Portugal present differences in //emm// types, superantigen gene content and antimicrobial resistance.</fc> BMC Microbiol. 12:280.[[http://www.ncbi.nlm.nih.gov/pubmed/23181337|(PMID: 23181337)]]

[[people:anacosta|C. Silva-Costa]], [[people:afriaes|A. Friães]], [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]], The Portuguese Group for the Study of Streptococcal Infections. 2012. <fc #0000FF>Differences between macrolide resistant and susceptible //Streptococcus pyogenes//: the importance of clonal properties in addition to antibiotic consumption.</fc> Antimicrob Agents Chemother. 56:5661–5666. [[http://www.ncbi.nlm.nih.gov/pubmed/22908153|(PMID: 22908153)]]

Thomas, V., A. Góis, B. Ritts, P. Burke, T. Hänscheid, and G. McDonnell. 2012. <fc #0000FF>A novel way to grow hemozoin-like crystals in vitro and its use to screen for hemozoin inhibiting antimalarial compounds.</fc> PLoS ONE 7:e41006. [[http://www.ncbi.nlm.nih.gov/pubmed/22815894|(PMID: 22815894)]]

Portuguese Laboratory Network for the Diagnosis of Influenza Infection. 2012. <fc #0000FF>Contribution of the Portuguese Laboratory Network for the Diagnosis of Influenza A(H1N1)pdm09 infection during the 2009/10 and 2010/11 influenza seasons.</fc> Euro Surveill. 17. [[http://www.ncbi.nlm.nih.gov/pubmed/22790607|(PMID: 22790607)]]

[[people:siaguiar|S. I. Aguiar]], [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2012. <fc #0000FF>Use of the 13-valent conjugate vaccine has the potential to eliminate pilus carrying isolates as causes of invasive pneumococcal disease.</fc> Vaccine 30:5487–5490. [[http://www.ncbi.nlm.nih.gov/pubmed/22749798|(PMID: 22749798)]]

[[http://www.biomedcentral.com/info/about/mostviewed/|{{:ha75.jpg|}}]] Francisco, A.P., C. Vaz, P. T. Monteiro, [[people:jmelocristino|J. Melo-Cristino]], [[people:ramirez|M. Ramirez]], and [[people:jcarrico|J. A. Carriço]]. 2012. <fc #0000FF>PHYLOViZ: Phylogenetic inference and data visualization for sequence based typing methods.</fc> BMC Bioinformatics, 13:87 [[http://www.ncbi.nlm.nih.gov/pubmed/22568821|(PMID: 22568821)]]

Frita, R., D. Carapau, M. M. Mota, and T. Hänscheid. 2012. <fc #0000FF>In vivo hemozoin kinetics after clearance of //Plasmodium berghei// infection in mice.</fc> Malar Res Treat 2012:373086. [[http://www.ncbi.nlm.nih.gov/pubmed/22567535|(PMID: 22567535)]]

Almeida, J., J. Tiple, [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]], C. Vaz, A. P. Francisco, and [[people:jcarrico|J. A. Carriço]]. 2012. <fc #0000FF>An Ontology and a REST API for Sequence Based Microbial Typing Data</fc>, p. 21–28. In A.T. Freitas, and A. Navarro (eds.), Bioinformatics for Personalized Medicine. Springer Berlin Heidelberg, Berlin, Heidelberg. [[http://dx.doi.org/10.1007/978-3-642-28062-7_3|DOI: 10.1007/978-3-642-28062-7_3]]

Rebelo, M., H. M. Shapiro, T. Amaral, [[people:jmelocristino|J. Melo-Cristino]], and T. Hänscheid. 2012. <fc #0000FF>Haemozoin detection in infected erythrocytes for //Plasmodium falciparum// malaria diagnosis-Prospects and limitations.</fc> Acta Tropica. 123:58-61. [[http://www.ncbi.nlm.nih.gov/pubmed/22465900|(PMID: 22465900)]]

[[people:emartins|Martins E. R.]], [[people:jmelocristino|J. Melo-Cristino]], [[people:ramirez|M. Ramirez]], Portuguese Group for the Study of Streptococcal Infections. 2012. <fc #0000FF>Dominance of serotype Ia among group B streptococci causing invasive infections in non-pregnant adults in Portugal.</fc> J Clin Microbiol. 50:1219–1227. [[http://www.ncbi.nlm.nih.gov/pubmed/22219307|(PMID: 22219307)]]

[[people:ahoracio|Horácio, A. N.]], [[people:jmiranda|J. Diamantino-Miranda]], [[people:siaguiar|S. I. Aguiar]], [[people:ramirez|M. Ramirez]], [[people:jmelocristino|J. Melo-Cristino]] and the Portuguese Group for the Study of Streptococcal Infections. 2012. <fc #0000FF>Serotype changes in adult invasive pneumococcal infections in Portugal did not reduce the high fraction of potentially vaccine preventable infections.</fc> Vaccine 30:218-224. [[http://www.ncbi.nlm.nih.gov/pubmed/22100892|(PMID: 22100892)]]

Mendes, J. J., A. Marques-Costa, C. Vilela, J. Neves, N. Candeias, P. Cavaco-Silva, and [[people:jmelocristino|J. Melo-Cristino]]. 2012. <fc #0000FF>Clinical and bacteriological survey of diabetic foot infections in Lisbon.</fc> Diabetes Res Clin Pract 95:153-161. [[http://www.ncbi.nlm.nih.gov/pubmed/22019426|(PMID: 22019426)]]

Narciso, A., F. Nunes, T. Amores, L. Lito, [[people:jmelocristino|J. Melo-Cristino]], and A. Duarte. 2012. <fc #0000FF>Persistence of uropathogenic //Escherichia coli// strains in the host for long periods of time: relationship between phylogenetic groups and virulence factors.</fc>  Eur J Clin Microbiol Infect Dis. 31:1211–1217. [[http://www.ncbi.nlm.nih.gov/pubmed/21990017|(PMID: 21990017)]]

===== 2011  =====

Costa, S. S., C. Falcao, M. Viveiros, D. Machado, M. Martins, [[people:jmelocristino|J. Melo-Cristino]], L. Amaral, and I. Couto. 2011. <fc #0000FF>Exploring the contribution of efflux on the resistance to fluoroquinolones in clinical isolates of //Staphylococcus aureus//</fc>. BMC Microbiol 11:241. [[http://www.ncbi.nlm.nih.gov/pubmed/22032541|(PMID: 22032541)]]

Carreiro, A. V., O. Anunciação, [[people:jcarrico|J. A. Carriço]], and S. C. Madeira. 2011. <fc #0000FF>Prognostic prediction through biclustering-based classification of clinical gene expression time series</fc>. J Integr Bioinform 8:175 [[http://www.ncbi.nlm.nih.gov/pubmed/21926438|(PMID: 21926438)]]

[[people:anaseveriano|Severiano A.]], F. R. Pinto, [[people:ramirez|M. Ramirez]], and [[people:jcarrico|J. A. Carriço]]. 2011. <fc #0000FF>Adjusted Wallace as a measure of congruence between typing methods</fc>. J Clin Microbiol 49:3997-4000 [[http://www.ncbi.nlm.nih.gov/pubmed/21918028|(PMID: 21918028)]]

[[people:emartins|Martins E. R.]], A. Andreu, P. Correia, T. Juncosa, J. Bosch, [[people:ramirez|M. Ramirez]], and [[people:jmelocristino|J. Melo-Cristino]] on behalf of the Microbiologist Group for the Study of Vertical Transmission Infections from the Catalan Society for Clinical Microbiology and Infectious Diseases. 2011. <fc #0000FF>Group B streptococci causing neonatal infections in Barcelona are a stable clonal population: 18-year surveillance</fc>. J Clin Microbiol. 49:2911-2918 [[http://www.ncbi.nlm.nih.gov/pubmed/21697333|(PMID: 21697333)]]

[[people:anaseveriano|Severiano A.]], [[people:jcarrico|J. A. Carriço]], D. A. Robinson, [[people:ramirez|M. Ramirez]], and F. R. Pinto. 2011. <fc #0000FF>Evaluation of jackknife and bootstrap for defining confidence intervals for pairwise agreement measures</fc>. PLoS ONE 6:e19539. [[http://www.ncbi.nlm.nih.gov/pubmed/21611165|(PMID: 21611165)]]

Hänscheid T., R. Romão, M. P. Grobusch, T. Amaral, and [[people:jmelocristino|J. Melo-Cristino]]. 2011. <fc #0000FF>Limitation of malaria diagnosis with the Cell-Dyn<sup>®</sup> analyser: not all haemozoin-containing monocytes are detected or shown</fc>. Int J Lab Hematol 33:e14-16. [[http://www.ncbi.nlm.nih.gov/pubmed/21457189|(PMID: 21457189)]]

Frita R., M. Rebelo, A. Pamplona, A. M. Vigario, M. M. Mota, M. P. Grobusch, and T. Hänscheid. 2011. <fc #0000FF>Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testing</fc>. Malar. J 10:74. [[http://www.ncbi.nlm.nih.gov/pubmed/   21453521
|(PMID:21453521)]]

Nordmann P, Picazo JJ, Mutters R, Korten V, Quintana A, Laeuffer JM, Seak JC, Flamm RK, Morrissey I; on behalf of the COMPACT study group. 2011. <fc #0000FF>Comparative activity of carbapenem testing: the COMPACT study</fc>. J Antimicrob Chemother. 66:1070-1078 [[http://www.ncbi.nlm.nih.gov/pubmed/21393160|(PMID: 21393160)]]

Hänscheid T., R. Badura, M. L. Fernandes, F. Antunes, and [[people:jmelocristino|J. Melo-Cristino]]. 2011. <fc #0000FF>The case of the disappearing mycobacteria in Ziehl-Neelsen-stained smears</fc>. Int. J. Infect. Dis 15:e291. [[http://www.ncbi.nlm.nih.gov/pubmed/21317005|(PMID: 21317005)]]

Sá-Leão R., F. Pinto, [[people:siaguiar|S. I. Aguiar]], S. Nunes, [[people:jcarrico|J. A. Carriço]], N. Frazão, N. Gonçalves-Sousa, [[people:jmelocristino|J. Melo-Cristino]], H. de Lencastre, and [[people:ramirez|M. Ramirez]]. 2011. <fc #0000FF>Analysis of invasiveness of pneumococcal serotypes and clones circulating in Portugal before widespread use of conjugate vaccines reveals heterogeneous behavior of clones expressing the same serotype</fc>. J. Clin. Microbiol 49:1369-1375. [[http://www.ncbi.nlm.nih.gov/pubmed/21270219|(PMID: 21270219)]]
===== 2010  =====

[[people:mcarrolo|Carrolo, M.]], [[people:mfrias|Frias, M. J.]], F. R. Pinto, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>Prophage spontaneous activation promotes DNA release enhancing biofilm formation in //Streptococcus pneumoniae//</fc>. PLoS ONE 5:e15678. [[http://www.ncbi.nlm.nih.gov/pubmed/21187931 |(PMID: 21187931)]]

Campuzano-Zuluaga G., T. Hanscheid, and M. P. Grobusch. 2010. <fc #0000FF>Automated haematology analysis to diagnose malaria</fc>. Malar. J. 9:346. [[http://www.ncbi.nlm.nih.gov/pubmed/21118557 |(PMID: 21118557)]]


[[people:ramirez|Ramirez M.]], and [[people:jmelocristino|J. Melo-Cristino]]. 2010. <fc #0000FF>Expanding the diagnosis of pediatric bacteremic pneumococcal pneumonia from blood cultures to molecular methods: advantages and caveats</fc>. Clin. Infect. Dis 51:1050-1052.[[http://www.ncbi.nlm.nih.gov/pubmed/20883111 |(PMID: 20883111)]]

Hänscheid T., and M. P. Grobusch. 2010. <fc #0000FF>Biosafety and tuberculosis laboratories in Africa</fc>. Lancet Infect. Dis. 10:582-583. [[http://www.ncbi.nlm.nih.gov/pubmed/20797636 |(PMID: 20797636)]]

McMillan D. J., D. E. Bessen, [[people:mdpinho|M. Pinho]], C. Ford, G. S. Hall, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>Population genetics of //Streptococcus dysgalactiae// subspecies //equisimilis// reveals widely dispersed clones and extensive recombination</fc>. PLoS ONE 5:e11741. [[http://www.ncbi.nlm.nih.gov/pubmed/20668530|(PMID: 20668530)]]

[[people:jmelocristino|Melo-Cristino J.]], L. Santos, [[people:anacosta|C. Silva-Costa]], [[people:afriaes|A. Friães]], [[people:mdpinho|M. D. Pinho]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>The Viriato Study: Update on antimicrobial resistance of microbial pathogens responsible for community-acquired respiratory tract infections in Portugal</fc>. Paediatr. Drugs 12:11-17. [[http://www.ncbi.nlm.nih.gov/pubmed/20590169|(PMID: 20590169)]]

[[people:siaguiar|Aguiar S. I.]], M. J. Brito, J. Gonçalo-Marques, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>Serotypes 1, 7F and 19A became the leading causes of pediatric invasive pneumococcal infections in Portugal after 7 years of heptavalent conjugate vaccine use</fc>. Vaccine. 28:5167-73 [[http://www.ncbi.nlm.nih.gov/pubmed/20558247|(PMID: 20558247)]]

[[people:mdpinho|Pinho M. D.]], [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>Fluoroquinolone resistance in //Streptococcus dysgalactiae// subsp. //equisimilis// and evidence for a shared global gene pool with //Streptoccocus pyogenes//</fc>. Antimicrob. Agents Chemother. 54:1769-1777. [[http://www.ncbi.nlm.nih.gov/pubmed/20145082 |(PMID: 20145082)]]

[[people:emartins|Martins E. R.]], [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2010. <fc #0000FF>Evidence for rare capsular switching in //Streptococcus agalactiae//</fc>. J. Bacteriol. 192:1361-1369 [[http://www.ncbi.nlm.nih.gov/pubmed/20023016|(PMID: 20023016)]]

Brilha S., H. Proença, [[people:jmelocristino|J. M. Cristino]], and T. Hänscheid. 2010. <fc #0000FF>Use of flow cytometry (Sysmex) UF-100) to screen for positive urine cultures: in search for the ideal cut-off</fc>. Clin. Chem. Lab. Med 48:289-292. [[http://www.ncbi.nlm.nih.gov/pubmed/19961394 |(PMID: 19961394)]]

Simões A. S., R. Sá-Leão, M. J. Eleveld, D. A. Tavares, [[people:jcarrico|J. A. Carriço]], H. J. Bootsma, and P. W. M. Hermans. 2010. <fc #0000FF>Highly penicillin-resistant multidrug-resistant pneumococcus-like strains colonizing children in Oeiras, Portugal: genomic characteristics and implications for surveillance</fc>. J. Clin. Microbiol. 48:238-46 [[http://www.ncbi.nlm.nih.gov/pubmed/19906899|(PMID: 19906899)]]

[[people:siaguiar|Aguiar S. I.]], F. R. Pinto, S. Nunes, I. Serrano, [[people:jmelocristino|J. Melo-Cristino]], R. Sá-Leão, [[people:ramirez|M. Ramirez]], and H. de Lencastre. 2010. <fc #0000FF>Increase of Denmark<sup>14</sup>-230 clone as a cause of pneumococcal infection in Portugal within a background of diverse serotype 19A lineages</fc>. J. Clin. Microbiol. 48:101–108. [[http://www.ncbi.nlm.nih.gov/pubmed/19864476|(PMID: 19864476)]]
===== 2009  =====
Hänscheid T., R. Frita, M. Längin, P. G. Kremsner, and M. P. Grobusch. 2009. <fc #0000FF>Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?</fc> Malar. J 8:255. [[http://www.ncbi.nlm.nih.gov/pubmed/19917088|(PMID: 19917088)]]

[[http://www.biomedcentral.com/info/about/mostviewed/|{{:ha75.jpg|}}]] [[people:mcarrolo|Carrolo, M.]], F. R. Pinto, [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2009. <fc #0000FF>Pherotypes are driving genetic differentiation within //Streptococcus pneumoniae//</fc>. BMC Microbiol 9:191. [[http://www.ncbi.nlm.nih.gov/pubmed/19735561|(PMID: 19735561)]]

Earnshaw S., D. L. Monnet, B. Duncan, J. O'Toole, K. Ekdahl, H. Goossens, European Antibiotic Awareness Day Technical Advisory Committee, and European Antibiotic Awareness Day Collaborative Group. 2009. <fc #0000FF>European Antibiotic Awareness Day, 2008 - the first Europe-wide public information campaign on prudent antibiotic use: methods and survey of activities in participating countries</fc>. Euro Surveill 14:19280. [[http://www.ncbi.nlm.nih.gov/pubmed/19643056|(PMID: 19643056)]]

[[people:mfrias|Frias, M. J.]], [[people:jmelocristino|J. Melo-Cristino]], and [[people:ramirez|M. Ramirez]]. 2009. <fc #0000FF>The autolysin LytA contributes to efficient bacteriophage progeny release in //Streptococcus pneumoniae//</fc>. J. Bacteriol. 191:5428-5440. [[http://www.ncbi.nlm.nih.gov/sites/entrez?term=19581370|(PMID: 19581370)]]

Francisco, A. P., M. Bugalho, [[people:ramirez|M. Ramirez]], and [[people:jcarrico|J. A. Carriço]]. 2009. <fc #0000FF>Global optimal eBURST analysis of multilocus typing data using a graphic matroid approach</fc>. BMC Bioinformatics 10:152. [[http://www.ncbi.nlm.nih.gov/sites/entrez?term=19450271|(PMID: 19450271)]]

Hänscheid T, Längin M, Codices V, Luty AJ, Adegnika AA, Kremsner PG, Grobusch MP. <fc #0000FF>Automated detection of haemozoin-containing monocytes for the diagnosis of malaria in microscopically negative cases during pregnancy</fc>. Acta Trop. 2009 Mar;109(3):245-6. [[http://www.ncbi.nlm.nih.gov/sites/entrez?term=19121281|(PMID: 19121281)]]

Pamplona A, Hanscheid T, Epiphanio S, Mota MM, Vigário AM. <fc #0000FF>Cerebral malaria and the hemolysis/methemoglobin/heme hypothesis: shedding new light on an old disease</fc>. Int J Biochem Cell Biol. 2009 Apr;41(4):711-6. [[http://www.ncbi.nlm.nih.gov/sites/entrez?term=18930163|(PMID: 18930163)]]

Hänscheid T, Valadas E. <fc #0000FF>Diagnosis of cryptosporidiosis using PCR or auramine O with LED fluorescent microscopy: which end of the stick?</fc> Acta Trop. 2009 Mar;109(3):247-8 (letter)

===== 2008  =====
Trigo, G., P. Ferreira, N. Ribeiro, M. Dinis, E. B. Andrade, J. Melo-Cristino, [[people:ramirez|M. Ramirez]], and D. Tavares. 2008. <fc #0000FF>Identification of immunoreactive extracellular proteins of //Streptococcus agalactiae// in bovine mastitis</fc>. Can. J. Microbiol. 54:899-905. [[http://www.ncbi.nlm.nih.gov/pubmed/18997846|(PMID: 18997846)]]

Pinto, F. R., J. Melo-Cristino, and [[people:ramirez|M. Ramirez]]. 2008. <fc #0000FF>A confidence interval for the Wallace coefficient of concordance and its application to microbial typing methods</fc>. PLoS ONE 3:e3696. [[http://www.ncbi.nlm.nih.gov/pubmed/19002246|(PMID: 19002246)]]

Aguiar, S. I., I. Serrano, F. R. Pinto, J. Melo-Cristino, [[people:ramirez|M. Ramirez]], and the Portuguese Surveillance Group for the Study of Respiratory Pathogens. 2008. <fc #0000FF>Changes in pneumococcal invasive disease with non-universal vaccination coverage of the seven-valent conjugate vaccine</fc>. Clin. Microbiol. Infect. 14:835-843. [[http://www.ncbi.nlm.nih.gov/pubmed/18844684|(PMID: 18844684)]]

Silva-Costa, C., F. R. Pinto, [[people:ramirez|M. Ramirez]], J. Melo-Cristino, and the Portuguese Surveillance Group for the Study of Respiratory Pathogens. 2008. <fc #0000FF>Decrease in macrolide resistance and clonal instability among //Streptococcus pyogenes// in Portugal</fc>. Clin. Microbiol. Infect. 14:1152-1159. [[http://www.ncbi.nlm.nih.gov/pubmed/19046174|(PMID: 19046174)]]

Pinto, F. R., S. I. Aguiar, J. Melo-Cristino, and [[people:ramirez|M. Ramirez]]. 2008. <fc #0000FF>Optimal control and analysis of two-color genomotyping experiments using bacterial multistrain arrays</fc>. BMC Genomics 9:230. [[http://www.ncbi.nlm.nih.gov/pubmed/18489741|(PMID: 18489741)]]

Aguiar, S. I., I. Serrano, F. R. Pinto, J. Melo-Cristino, and [[people:ramirez|M. Ramirez]]. 2008. <fc #0000FF>The presence of the pilus locus is a clonal property among pneumococcal invasive isolates</fc>. BMC Microbiol 8:41. [[http://www.ncbi.nlm.nih.gov/pubmed/18307767|(PMID: 18307767)]]

Faria, N. A., J. A. Carrico, D. C. Oliveira, [[people:ramirez|M. Ramirez]], and H. de Lencastre. 2008. <fc #0000FF>Analysis of typing methods for epidemiological surveillance of both methicillin-resistant and methicillin-susceptible //Staphylococcus aureus// strains</fc>. J. Clin. Microbiol. 46:136-144. [[http://www.ncbi.nlm.nih.gov/pubmed/17989188|(PMID: 17989188)]]

Tavares, A., J. Inacio, J. Melo-Cristino, and I. Couto. 2008. <fc #0000FF>Use of fluorescence //in situ// hybridization for rapid identification of staphylococci in blood culture samples collected in a Portuguese hospital</fc>. J Clin Microbiol 46:3097-3100.

Leitão, J. H., S. A. Sousa, M. V. Cunha, M. J. Salgado, J. Melo-Cristino, M. C. Barreto, and I. Sa-Correia. 2008. <fc #0000FF>Variation of the antimicrobial susceptibility profiles of //Burkholderia cepacia// complex clonal isolates obtained from chronically infected cystic fibrosis patients: a five-year survey in the major Portuguese treatment center</fc>. Eur J Clin Microbiol Infect Dis 27:1101-1111

Hanscheid T, Melo Cristino J, Salgado MJ.<fc #0000FF> Screening of auramine stained smears of all fecal samples is a rapid and inexpensive way to increase the detection of coccidial infections</fc>. 2008. Int J Infect Dis; 12(1):47-50. 

Barbosa JM, Costa-de-Oliveira S, Rodrigues AG, Hanscheid T, Shapiro H, Pina-Vaz C. <fc #0000FF>A flow cytometric protocol for detection of //Cryptosporidium// spp.</fc> 2008. Cytometry A; 73(1):44-7. 

Hänscheid T. <fc #0000FF>The future looks bright: low-cost fluorescent microscopes for detection of //Mycobacterium tuberculosis// and //Coccidiae//.</fc> 2008. Trans R Soc Trop Med Hyg; 102:520-1. 

Shapiro HM, Hänscheid T. <fc #0000FF>Fuchsin fluorescence in //Mycobacterium tuberculosis//: The Ziehl-Neelsen stain in a new light</fc>. 2008. J Microbiol Methods; 74: 119-120. 

Vicente A, Barros R, Florinda A, Silva A, Hanscheid T. 2008. <fc #0000FF>High rates of fluoroquinolone-resistant Campylobacter in Portugal--need for surveillance</fc>. Euro Surveill; 13. pii: 8031. 

===== 2007  =====

Hänscheid T, Längin M, Lell B, Pötschke M, Oyakhirome S, Kremsner PG, Grobusch MP. 2008. <fc #0000FF>Full blood count and haemozoin-containing leukocytes in children with malaria: diagnostic value and association with disease severity</fc>. Malar J  12; 7:109. 

Hänscheid T, Ribeiro CM, Shapiro HM, Perlmutter NG.  <fc #0000FF>Fluorescence microscopy for tuberculosis diagnosis</fc>. Lancet Infect Dis 7: 236-237.2007. 

Hänscheid T, Egan TJ, Grobusch MP. <fc #0000FF>Haemozoin: from melatonin pigment to drug target, diagnostic tool, and immune modulator</fc>. Lancet Infect Dis 7: 675-85. 2007.

M.V. Cunha, A. Pinto-de-Oliveira, L. Meirinho-Soares, M.J. Salgado, J. Melo-Cristino, S. Correia, C. Barreto, I. Sá-Correia. <fc #0000FF>Excepcionally high representation of //Burkholderia cepacia// among //B. cepacia// complex isolates recovered from the major Portuguese Cystic Fibrosis Center</fc>. J  Clin Microbiol 45: 1628-1633, 2007. 

A. Friães, [[people:ramirez|M. Ramirez]], J. Melo-Cristino and the Portuguese group for the study of streptococcal infections. <fc #0000FF>Non-outbreak surveillance of group A streptococci causing invasive disease in Portugal identified internationally disseminated clones among members of a genetically heterogeneous population</fc>. J Clin Microbiol 45: 2044-2047, 2007.  [[http://www.ncbi.nlm.nih.gov/pubmed/17460058|(PMID: 17460058)]]

E.R. Martins, M.A. Pessanha, [[people:ramirez|M. Ramirez]], J. Melo-Cristino and the Portuguese Group for the Study of Streptococcal Infections. <fc #0000FF>Analysis of Group B streptococcal isolates from infants and pregnant women in Portugal revealing two lineages with enhanced invasiveness</fc>. J Clin Microbiol 45: 3224-3229, 2007. [[http://www.ncbi.nlm.nih.gov/pubmed/17699641|(PMID: 17699641)]]

E.R. Martins, J. Melo-Cristino, [[people:ramirez|M. Ramirez]]. <fc #0000FF>Reevaluating the serotype II capsular locus of //Streptococcus agalactiae//</fc>. J  Clin Microbiol 45: 3384-3386, 2007.   [[http://www.ncbi.nlm.nih.gov/pubmed/17715372|(PMID: 17715372)]]

E.R. Martins, C. Florindo, F. Martins, I. Aldir, M.J. Borrego, L. Brum, [[people:ramirez|M. Ramirez]], J. Melo-Cristino. <fc #0000FF>//Streptococcus agalactiae// serotype Ib as an agent of meningitis in two adult non-pregnant women</fc>. J Clin Microbiol 45: 3850-3852, 2007.   [[http://www.ncbi.nlm.nih.gov/pubmed/17881554|(PMID: 17881554)]]

Pinto, F. R., J. A. Carriço, [[people:ramirez|M. Ramirez]], and J. S. Almeida. 2007. <fc #0000FF>Ranked Adjusted Rand: integrating distance and partition information in a measure of clustering agreement</fc>. BMC Bioinformatics 8:44.   [[http://www.ncbi.nlm.nih.gov/pubmed/17286861|(PMID: 17286861)]]

===== 2006  =====

Pinho, M. D., J. Melo-Cristino, [[people:ramirez|M. Ramirez]], the Portuguese group for the study of streptococcal infections. 2006. <fc #0000FF>Clonal relationships between invasive and noninvasive lancefield group C and G Streptococci and //emm//-specific differences in invasiveness</fc>. J. Clin. Microbiol. 44:841-846.  [[http://www.ncbi.nlm.nih.gov/pubmed/16517864|(PMID: 16517864)]]

Silva-Costa, C., [[people:ramirez|M. Ramirez]], J. Melo-Cristino. 2006. <fc #0000FF>Identification of macrolide-resistant clones of //Streptococcus pyogenes// in Portugal</fc>. Clin. Microbiol. Infect. 12:513-518. [[http://www.ncbi.nlm.nih.gov/pubmed/16700698|(PMID: 16700698)]]

Carriço, J.A., C. Silva-Costa, J. Melo-Cristino, F. R. Pinto, H. de Lencastre, J.S. Almeida, [[people:ramirez|M. Ramirez]]. 2006. <fc #0000FF>Illustration of a common framework for relating multiple typing methods by application to macrolide- resistant //Streptococcus pyogenes//</fc>. J. Clin. Microbiol. 44:2524-2532. [[http://www.ncbi.nlm.nih.gov/pubmed/16825375|(PMID: 16825375)]]

Serrano, I., J. Melo-Cristino, [[people:ramirez|M. Ramirez]]. 2006. <fc #0000FF>Heterogeneity of pneumococcal phase variants in invasive human infections</fc>. BMC Microbiol. 6:67. [[http://www.ncbi.nlm.nih.gov/pubmed/16872486|(PMID: 16872486)]]

Aguiar, S. I., M. J. Frias, L. Santos, J. Melo-Cristino, and [[people:ramirez|M. Ramirez]]. 2006. <fc #0000FF>Emergence of optochin resistance among //Streptococcus pneumoniae// in Portugal</fc>. Microb. Drug Resist. 12:239-245. [[http://www.ncbi.nlm.nih.gov/pubmed/17227208|(PMID: 17227208)]]

Figueiredo, T. A., S. I. Aguiar, J. Melo-Cristino, and [[people:ramirez|M. Ramirez]]. 2006. <fc #0000FF>DNA methylase activity as a marker for the presence of a family of phage-like elements conferring efflux-mediated macrolide resistance in streptococci</fc>. Antimicrob. Agents Chemother. 50:3689-3694. [[http://www.ncbi.nlm.nih.gov/pubmed/16954322|(PMID: 16954322)]]

Hanscheid T, Valadas E. <fc #0000FF>Adequately performed stool ova and parasite examinations: is the best the enemy of the good?</fc> Clin Infect Dis. 2006;43:117-8. 

===== 2005  =====

Silva-Costa, C., [[people:ramirez|M. Ramirez]], and J. Melo-Cristino. 2005. <fc #0000FF>Rapid inversion of the prevalences of macrolide resistance phenotypes paralleled by a diversification of T and //emm// types among Streptococcus pyogenes in Portugal</fc>. Antimicrob. Agents Chemother. 49:2109-2111. [[http://www.ncbi.nlm.nih.gov/pubmed/15855540|(PMID: 15855540)]]

Serrano, I., J. Melo-Cristino, J. A. Carriço, and [[people:ramirez|M. Ramirez]]. 2005. <fc #0000FF>Characterization of the genetic lineages responsible for pneumococcal invasive disease in Portugal</fc>. J. Clin. Microbiol. 43:1706-1715. [[http://www.ncbi.nlm.nih.gov/pubmed/15814989|(PMID: 15814989)]]

 Hanscheid T, Monteiro C, Cristino JM, Lito LM, Salgado MJ. 2005. <fc #0000FF>Growth of //Mycobacterium tuberculosis// in conventional BacT/ALERT FA blood culture bottles allows reliable diagnosis of Mycobacteremia</fc>. J. Clin. Microbiol. 43:890-891. 

T. Conceição, A. Brizio, A. Duarte, L. M. Lito, J. Melo Cristino, M.J. Salgado. <fc #0000FF>First description of CTX-M-15-producing //Klebsiella pneumoniae// in Portugal</fc>. Antimicrobial Agents and Chemotherapy 49: 477-478, 2005.

===== 2004  =====

Conceição, T., N. Faria, L. Lito, J. Melo Cristino, M. J. Salgado, and A. Duarte. 2004. Diversity of chromosomal AmpC beta-lactamases from Enterobacter cloacae isolates in a Portuguese hospital. FEMS Microbiol Lett 230:197-202.

Conceição, T., N. Faria, M. Pimentel, G. Soveral, A. Duarte, L. M. Lito, J. Melo Cristino, and M. J. Salgado. 2004. New chromosomal AmpC beta-lactamase in Enterobacter cloacae. Antimicrob Agents Chemother 48:1437.

Figueira-Coelho, J., [[people:ramirez|M. Ramirez]], M. J. Salgado, and J. Melo-Cristino. 2004. Streptococcus agalactiae in a large Portuguese teaching hospital: antimicrobial susceptibility, serotype distribution, and clonal analysis of macrolide-resistant isolates. Microb Drug Resist 10:31-36. [[http://www.ncbi.nlm.nih.gov/pubmed/15140391|(PMID: 15140391)]]

Hänscheid, T., C. Monteiro, L. Marques-Lito, J. Melo-Cristino, and M. J. Salgado. 2004. Usefulness of Myco/F Lytic blood cultures (Bactec 9050) in the detection of Mycobacterium tuberculosis bacteraemia in HIV-infected patients in Portugal. Int J Infect Dis 8:253-254.

Serrano, I., [[people:ramirez|M. Ramirez]], The Portuguese Surveillance Group for the Study of Respiratory Pathogens, and J. Melo-Cristino. 2004. Invasive Streptococcus pneumoniae from Portugal: implications for vaccination and antimicrobial therapy. Clin Microbiol Infect 10:652-656. [[http://www.ncbi.nlm.nih.gov/pubmed/15214879|(PMID: 15214879)]]

Valadas, E., T. Hänscheid, and F. Antunes. 2004. HIV Infection and non-tuberculous mycobacteria: how important in southern European countries? Scand J Infect Dis 36:685-686.

Zé-Zé, L., R. Tenreiro, A. Duarte, M. J. Salgado, J. Melo-Cristino, L. Lito, M. M. Carmo, S. Felisberto, and G. Carmo. 2004. Case of aortic endocarditis caused by Lactobacillus casei. J Med Microbiol 53:451-453.

===== 2003  =====

J. Melo Cristino, [[people:ramirez|M. Ramirez]], N. Serrano, T. Hänscheid, and The Portuguese Surveillance Group for the Study of Respiratory Pathogens. 2003. Macrolide resistance in Streptococcus pneumoniae isolated from patients with community-acquired lower respiratory tract infections in Portugal: Results of a 3-year (1999-2001) multicenter surveillance study Microbial Drug Resistance 9: 73-80. [[http://www.ncbi.nlm.nih.gov/pubmed/12705685|(PMID: 12705685)]]

T. Hänscheid, B.G. Pinto, J. Melo Cristino, M.P. Grobusch. 2003. Avoiding misdiagnosis of imported malaria: screening of emergency room samples with thrombocytopenia detects clinically unsuspected cases. Journal of Travel Medicine 10: 155-157.

A. Duarte, F. Boavida, F. Grosso, M. Correia, L.M. Lito, J. Melo Cristino, M.J. Salgado. Outbreak of GES-1 beta-Lactamase-Producing Multidrug-Resistant Klebsiella pneumoniae in a University Hospital in Lisbon, Portugal. Antimicrob Agents Chemother 47:1481-1482.

M. Correia, F. Boavida, F. Grosso, M.J. Salgado, L.M. Lito, J. Melo Cristino, S. Mendo, A. Duarte. 2003. Molecular characterization of a new class 3 integron in Klebsiella pneumoniae”. Antimicrob Agents Chemother 47:2838-2843, 2003.

Grobusch PM, Hänscheid T, Göbels K, Slevogt H, Zoller T, Rögler G, Teichmann D. Comparison of three antigen detection tests for diagnosis and follow-up of falciparum malaria in travellers returning to Berlin, Germany. Parasitol Res 2003; 89: 354-357

Hänscheid T. 2003. Update: Current strategies to avoid misdiagnosis of malaria. Clin Microbiol Infect 203; 997-504

Grobusch MP, Hänscheid T, Krämer B, Neukammer J, May J, Seybold J, Kun JFJ, Suttorp N. 2003. Diagnosis of malaria by automated detection of malaria pigment in travelers returning to Berlin, Germany. Cytometry 55B(1):46-51.

Valadas E, T, Fernandes ML, Antunes F. 2003. Smear microsocopy to diagnose tuberculosis early and prevent further transmission in a population with a high prevelance of HIV infection. Clin Microbiol Infect 2003; 9(10):1045-1047

Brito, D. A., [[people:ramirez|M. Ramirez]] and H. de Lencastre. 2003. Serotyping Streptococcus pneumoniae by multiplex PCR. J. Clin. Microbiol. 41:2378-2384.  [[http://www.ncbi.nlm.nih.gov/pubmed/12791852|(PMID: 12791852)]]